pathophysiological effect of Pegensynthetic dye On Rabbit(Oryctolaguscuniculus) Renal and liver functions

الملخص

Pegen is a type of commercial synthetic dye used for hair
and skin ,it is toxic chemical compound which contains
Paraphenylenediamine (PPD) mostlyharmful when the exporters
were repeated either by inhalation or absorption and affected the
physiology of human and animals systems,Moreover,this study
was constructed to find physiological effects on blood parameters
,liver and the kidneys of the rabbits(Oryctolagus cuniculus).
Thirty sixexperimental laboratory animalswere used to fulfil this
study and divided into three groups,each of 12 animals, first one
is control, while the second for application of natural henna and
the third one for Pegen synthetic dye at intervals of 10 days for
six months .
Thin Layer Chromatography (TLC) and High-Performance
Liquid Chromatography (HPLC) were used for defecting and
determining the concentration of PPD in Pegen(0.187mg/l).
Blood samples were collected every two months for tests of
kidney and liver functions and haematological indicesthe red blood countswere measured by Sysmex-KX which recorded low
values of 3.92 ×106/ul compared to control which found to be
5.16×106/ul. The results of haemoglobin concentration
(9.00g/dl))similarly were showed significant slightdecrease
compared to the control for 11.42mg/dlwhereas leukocyte count
with value6.89 x103/ul were record significant increase to the
value 9.55x103/ul. Also, liver test for GOT GPT ALT AST
ALP/ul,T.P, Alb and Bili mg/dlof
values105.80,86.40,86.60,350.74,6.69,5.60,6.17 and 1.29 were
showed abnormality compared to control values of 37.40,19.70
,67.46 ,297.20 ,5.05 ,7.45,3.42 and 0.42 respectively .
On other hand, kidney functiontestswere found to be abnormal in
those who used Pegen such as blood urea, Creatinine Potassium,
and Sodium for values 1.81, 57.43, 6.19 and120.50 compared to
the natural henna values of 0.64,29.60,5.22 and 142.15
respectively (p<0.05).